This study was performed to find the most effective antibactrial agent for the treatment of Vibrio vulnificus septicemia.
By a standardized broth method, minimum inhibitory concentrations(MICs) and minimum bactericidal concentrations (MBCs) were determined for 28 strains of Vibrio vulnificus. MICs and MBC of tetracycline (TC), ampicillin (AP), chloramphenicol (CM)
and
the
quinolones such as ciprofloxacin (CX) , norfloxacin (NX), pefloxacin (PX), pipemidic acid, nalidixic acid, and oxolinic acid were very low. However, aminoglycosides and cephalosporins showed very poor antibacterial effects. When MICs were
compared
each
other, AP, CM and TC had the same values of the two in 17-39%, whereas the quinolones had in 64-86%. In order to compare the bactericidal effect of quinolones and TC which is known as bactericidal agent against Vibrio vulnificus, cultured cells
were
treated with 2×MIC for 3 hours. The survival rate of CX-and NX-treated cell was less than 0.01%, showing excellent bactericidal effect, and those of PX-treated cells and TC treated cells were 0.03% and 0.16%, respectively. For further
investigation
into the bactericidal activity of TC and CX, the bacterial cultures in stationary phase and log phase were treated with TC or CX, respectively. During the stationary phase, CX showed 90% bactericidal effect within 1 hour but TC within 4 hours.
During
the log phase, CX showed 99% bactericidal effect within 1.5 hours TC within 2-3 hours.
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